ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
Objective To observe the larvicidal activities of transgenic cyanobacteria with CryIVD gene of Bacillus thuringiensis subsp. israelensis against different larval instars of Culex pipiens pallens. Methods Transgenic cyanobacteria with CryIVD gene of Bacillus thuringiensis subsp. israelensis were cultured in large numbers and were then dried and stored. The larvicidal activities of transgenic cyanobacteria stored for different periods were determined against different larval instars of Cx. pipiens pallens.Results The dried and stored transgenic cyanobacteria demonstrated a high toxicity to Cx. pipiens pallens larvae, and the longer the dried ones were stored, the lower their toxicity was. Compared with fresh ones, the stored transgenic cyanobacteria had slightly decreased larvicidal activity. They killed over 50% of larvae of different instars in 48 hours at concentration of 105 cells/ml. At a concentration of 106 cells/ml, transgenic cyanobacteria stored for one year, two years, and three years showed similar larvicidal activities, resulting in no significant differences in mortality rate of Cx. pipiens pallens larvae (F=9.32, P>0.01), but there was significant difference in the mortality rate when comparison was made between the transgenic cyanobacteria stored for one year and three years at a concentration of 105 cells/ml (F=101.21, P<0.01). Conclusion The cryIVD gene of Bacillus thuringiensis subsp. israelensis and larvicidal activity of stored transgenic cyanobacteria remain stable, and the stored transgenic cyanobacteria provide a convenient way for use in the field.
Prophenoloxidase-activating system is an important part in the immune system of Musca domestica and plays an essential role in the pathogen recognition and immune defense. Until now, however, the key genes in the prophenoloxidase-activating system and the system's action mechanism remain unclear. On this occasion, we performed transcriptomic analysis of M. domestica to obtain a number of expressed sequence tags of the key genes of prophenoloxidases, prophenoloxidase-activating enzymes, serine proteinase inhibitors (Serpin), and upstream pattern-recognition receptors in the prophenoloxidase-activating system. This article reviews the research advances in the key genes in the prophenoloxidase-activating system of M. domestica.
【Abstract】 Objective To explore the effect of frozen storage on non-specific esterase (NSE) activity of Culex pipiens pallens and provide a basis for the generalization and application of insecticide-resistance detection of mosquito by biochemical method. Methods The NSE activity of single mosquito was determined based on β-naphthalene acetic acid ester (β-naphthyl acetate, β-NA) as substrate and solid blue B salt (fast blue B salt) as developer. Results The NSE activity of DDVP-resistant strain was obviously higher than that of susceptible strain. For the A value of NSE activity from Cx.pipiens pallens after frozen for 0, 7, 14, 21 and 28 days at -20 ℃, the F value of 4 instar larvae of susceptible strain mosquito was 0.7642 by the complete randomized design variance comparison and the F value of 3-day-old adult female of susceptible strain mosquito was 0.7452. The F value of 4 instar larvae of DDVP resistant strain mosquito was 0.1092, and the F value of 3-day-old adult female of DDVP-resistant strain mosquito was 0.1521. All of the P value were greater than 0.05 and no significant difference was found. Conclusion There was no difference between on the NSE activity of 4 instar larvae and 3-day-old adult female of Cx.pipiens pallens after frozen for 28 days, and mosquitoes collected from the field could be taken to the lab for batch determination.